Abstract
Wild grapevine (Vitis vinifera subsp. sylvestris) is widely recognized as an important source of resistance or tolerance genes for diseases and environmental stresses. Recent studies revealed partial resistance to powdery mildew (Erysiphe necator, PM) in V. sylvestris from Central Asia. Here, we report resistance to PM of V. sylvestris collected from different regions of Croatia and in seedling populations established from in situ V. sylvestris accessions. Ninety-one in situ individuals and 67 V. sylvestris seedlings were evaluated for PM resistance according to OIV 455 descriptor. Three SSR markers (SC47-18, SC8-071-0014, and UDV-124) linked to PM resistance locus Ren1 were used to decipher allelic structure. Nine seedlings showed resistance in in vivo evaluations while leaf disk assays revealed three PM-resistant accessions. One V. vinifera cultivar used as a control for PM evaluations also showed high phenotypic resistance. Based on the presence of one or two resistance alleles that are linked to the Ren1 locus, 32 resistant seedlings and 41 resistant in situ genotypes were identified in the investigated set. Eight seedlings showed consistent phenotypic PM resistance, of which seven carried one or two alleles at the tested markers. This study provides the first evidence of PM resistance present within the eastern Adriatic V. sylvestris germplasm.
Highlights
Wild grapevine (Vitis vinifera subsp. sylvestris) is widely recognized as an important source of resistance or tolerance genes for diseases and environmental stresses
Detailed evaluation for powdery mildew (PM) resistance continued on 67 V. sylvestris seedlings grown in pots in an ex-situ seedling collection
This study represents the first screening for powdery mildew resistance in eastern Adriatic V. sylvestris germplasm
Summary
Wild grapevine (Vitis vinifera subsp. sylvestris) is widely recognized as an important source of resistance or tolerance genes for diseases and environmental stresses. Recent studies revealed partial resistance to powdery mildew (Erysiphe necator, PM) in V. sylvestris from Central Asia. One V. vinifera cultivar used as a control for PM evaluations showed high phenotypic resistance. Eight seedlings showed consistent phenotypic PM resistance, of which seven carried one or two alleles at the tested markers. Powdery mildew (Erysiphe necator) is an economically important fungal disease of grapevine. It has been a continuous problem since its introduction from North America to Europe around 1845. After the onset of this disease in Europe, inorganic fungicides and sulfur were used to control powdery mildew (PM). PM, including exposure of the fungus to UV light at night, when the fungal defense system ‘turns off ’5 Another solution comes from the grapevine genome itself.
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