Abstract
Using agarose gel chromatography, RNA with characteristics of double-stranded molecules was isolated from KB cells infected with type 2 adenovirus. Results of studies measuring its susceptibility to a number of nuclease activities were consistent with its identification as double-stranded RNA. The material was resistant to digestion by both pancreatic and T1 ribonuclease and by pancreatic deoxyribonuclease; and after heating at 100°C, it was much more sensitive to ribonuclease hydrolysis. Moreover, nearly 30% of the RNA formed a ribonuclease-resistant hybrid when denatured and incubated with purified type 2 adenovirus DNA, indicating that at least a portion of the double-stranded RNA in infected KB cells was transcribed from the viral genome.
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