RNA production in adenovirus-infected KB cells

Abstract

Ribonucleic acid (RNA) from adenovirus type 2-infected KB cells was examined with DNA-RNA homology techniques ( Nygaard and Hall, 1963), sucrose gradient analysis and methylated albumin column chromatography. The presence of viral complementary RNA was detected as early as 9 hours after infection, and relatively larger amounts were present 28 hours after infection. Although sucrose gradient analysis revealed no differences in radioactive labeling of RNA from infected and uninfected KB cells, an unusual nucleic acid component appeared in RNA from infected KB cells when analysis was performed with methylated albumin column chromatography. This component was absent from uninfected cell cultures.