Abstract
BackgroundThe disability rate associated with rheumatoid arthritis (RA) ranks high among inflammatory joint diseases. However, the cause and potential molecular events are as yet not clear. Here, we aimed to identify differentially expressed genes (DEGs), pathways and immune infiltration involved in RA utilizing integrated bioinformatics analysis and investigating potential molecular mechanisms.Materials and methodsThe expression profiles of GSE55235, GSE55457, GSE55584 and GSE77298 were downloaded from the Gene Expression Omnibus database, which contained 76 synovial membrane samples, including 49 RA samples and 27 normal controls. The microarray datasets were consolidated and DEGs were acquired and further analyzed by bioinformatics techniques. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DEGs were performed using R (version 3.6.1) software, respectively. The protein-protein interaction (PPI) network of DEGs were developed utilizing the STRING database. Finally, the CIBERSORT was used to evaluate the infiltration of immune cells in RA.ResultsA total of 828 DEGs were recognized, with 758 up-regulated and 70 down-regulated. GO and KEGG pathway analyses demonstrated that these DEGs focused primarily on cytokine receptor activity and relevant signaling pathways. The 30 most firmly related genes among DEGs were identified from the PPI network. The principal component analysis showed that there was a significant difference between the two tissues in infiltration immune.ConclusionThis study shows that screening for DEGs, pathways and immune infiltration utilizing integrated bioinformatics analyses could aid in the comprehension of the molecular mechanisms involved in RA development. Besides, our study provides valuable data related to DEGs, pathways and immune infiltration of RA and may provide new insight into the understanding of molecular mechanisms.
Highlights
Rheumatoid arthritis (RA) occurs in approximately 5 per 1000 people and can inevitably prompt severe joint damage and disability
Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses demonstrated that these differentially expressed genes (DEGs) focused primarily on cytokine receptor activity and relevant signaling pathways
This study shows that screening for DEGs, pathways and immune infiltration utilizing integrated bioinformatics analyses could aid in the comprehension of the molecular mechanisms involved in rheumatoid arthritis (RA) development
Summary
Rheumatoid arthritis (RA) occurs in approximately 5 per 1000 people and can inevitably prompt severe joint damage and disability. The disability rate of RA ranks high among the arthritic which occurs in multiple-joint on the human body, and the incidence of this kind of arthritis is increasing year by year. RA would lead to multiple-joint dysfunction, disability, lower quality of life, respiratory illness, cardiovascular disease, and other comorbidities in patients not receiving intervention [2]. The etiology of RA is still ambiguous All things considered, both genetic factors and environmental factors, contribute to the occurrence and development of RA [3]. The disability rate associated with rheumatoid arthritis (RA) ranks high among inflammatory joint diseases. We aimed to identify differentially expressed genes (DEGs), pathways and immune infiltration involved in RA utilizing integrated bioinformatics analysis and investigating potential molecular mechanisms
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
