Identification of different faces of Pseudomonas aeruginosa isolates in burn patients by genetic fingerprinting

Abstract

Pseudomonas aeruginosa, as a prevalent infection in hospital settings, has a different form of performance and response to anti-bacterial agents. Categorizing of infections based on their genome structure may be helpful in control and treatment of prevalent infections. Total of 278 burn patients were evaluated (March 2016 to March 2017) at Amir-Al-Momenin burn hospital (SUMS) for P. aeruginosa by standard microbiological tests. Confirmed isolates were categorized by RAPD-PCR technique following the evaluation for antibacterial susceptibility tests. RAPD finger print results were analyzed by Gelj software (V.2). Based on the experiment results total of 47 (33.1%) P. aeruginosa were isolated. According to the antibiogram results, >38% were resistant to all available antimicrobials except Colistin, while 32% were multi-drug resistant. In RAPD results, 8 patterns were identified with primer 272 and 5 with 277. Patterns 4, 2 in primer 272 and 4, 3 in primer 272 were the most prevalent patterns. Results showed that primer 277 had more power in categorizing the isolates for defining antibiotic sensitive pattern. In this category, sensitive isolates (>95%) were in patterns 2 and 3, while more resistant isolates (>90%) were in patterns 4 and 5. Based on the results of the study, it was found that fingerprinting with primer 272 had the ability to categorize P. aeruginosa isolates for introducing antibiotic pattern, while this result must be confirmed by more different species and techniques in other centers.