Abstract
Olfaction plays an indispensable role in mediating insect behavior, such as locating host plants, mating partners, and avoidance of toxins and predators. Olfactory-related proteins are required for olfactory perception of insects. However, very few olfactory-related genes have been reported in Plodia interpunctella up to now. In the present study, we sequenced the antennae transcriptome of P. interpunctella using the next-generation sequencing technology, and identified 117 candidate olfactory-related genes, including 29 odorant-binding proteins (OBPs), 15 chemosensory proteins (CSPs), three sensory neuron membrane proteins (SNMPs), 47 odorant receptors (ORs), 14 ionotropic receptors (IRs) and nine gustatory receptors (GRs). Further analysis of qRT-PCR revealed that nine OBPs, three CSPs, two SNMPs, nine ORs and two GRs were specifically expressed in the male antennae, whereas eight OBPs, six CSPs, one SNMP, 16 ORs, two GRs and seven IRs significantly expressed in the female antennae. Taken together, our results provided useful information for further functional studies on insect genes related to recognition of pheromone and odorant, which might be meaningful targets for pest management.
Highlights
Indian meal moth, Plodia interpunctella (Hubener) (Lepidoptera: Pyraloidea, Pyralidae), is a notorious stored-product pest worldwide [1]
Sequence analysis and assembly cDNA library of Plodia interpunctella was constructed using the TRINITY de novo assembly program, and short-read sequences were assembled into 150,633 transcripts with a mean length of 1,491 bp and an N50 of 3,567 bp
The raw reads of P. interpunctella transcriptome have been deposited into the NCBI SRA database, and the Transcriptome Shotgun Assembly (TSA) project has been deposited at DDBJ/ENA/GenBank under the accession GFWQ00000000
Summary
Plodia interpunctella (Hubener) (Lepidoptera: Pyraloidea, Pyralidae), is a notorious stored-product pest worldwide [1]. The larvae infest a variety of processed foods, including fruits, nuts, cereals, powdered milk, chocolate, birdseed, and pet food [2], causing extensive damage by impairing dry weight, germination, nutritional value, and quality grade. It is difficult to control P. interpunctella by conventional insecticides, because it often inhabits our kitchen, closet and warehouse, and its larvae are mixed with our processed foods. Several novel strategies have been developed to monitor and control P. interpunctella. Among these novel methods, sex pheromone is widely acceptable due to its safety and efficiency. Host volatiles have been thought to affect the oviposition behavior of P
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