Identification and expression profiling of the Aux/IAA gene family in Chinese hickory (Carya cathayensis Sarg.) during the grafting process

Abstract

Auxin is an essential regulator in various aspects of organism growth and development. Members of the Aux/IAA family of genes encode short-lived nuclear proteins and mediate the responses of auxin-regulated gene expression. Here, the first identification and characterization of 22 cDNAs encoding the open reading frame of the Aux/IAA family in Chinese hickory (named as CcIAA) has been performed. The proteins encoded by these genes contain four whole or partially conserved domains of the Aux/IAA family. Phylogenetic analysis indicated that CcIAAs were unevenly distributed among eight different subgroups. The spatio-specific expression profiles showed that most of the CcIAAs preferentially expressed in specific tissues. Three CcIAA genes, including CcIAA11, CcIAA27a2 and CcIAAx, were predominantly expressed in stem. The predominant expression of CcIAA genes in stems might play important roles in vascular reconnection during the graft process. Furthermore, expression profiles of Aux/IAA genes during the grafting process of Chinese hickory have been analysed. Our data suggested that 19 CcIAAs were down-regulated and 3 CcIAAs (including CcIAA28, CcIAA8a and CcIAA27b) were induced, indicating their specializations during the grafting process. The involvement of CcIAA genes at the early stage after grafting gives us an opportunity to understand the role of auxin signalling in the grafting process.