Abstract
Two acetylated 4α-methylsterols were isolated as an inseparable mixture from the octocoral Psuedoplexaura flagellosa. By the use of concerted 2D NMR (COSY, HSQC, HMBC and T-ROESY) techniques, it was possible to both identify the compounds as the acetates of 4α, 24R-dimethyl-5α-cholest-22-en-3β-ol and 4α, 23, 24R-trimethyl-5α-cholest-22-en-3-β-ol and to assign fully their 1H and 13C spectra. The general usefulness of 2D NMR for identifying individual components of complex mixtures of natural products is discussed. Copyright © 2001 John Wiley & Sons, Ltd.
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