Abstract
In the present study, a simple, sensitive, and rapid hydrophilic interaction chromatographic (HILIC) method with ultraviolet (UV) detection for the analysis of oxytetracycline (OTC) was developed and applied to the determination of the antibiotic in environmental waters. OTC was extracted and purified from water samples by solid-phase extraction (SPE) employing Oasis HLB cartridge and analyzed on a Kromasil silica column with the mobile phase of acetonitrile-sodium oxalate (10 mM, pH 2.5) (90:10, v/v). The analyte was detected at 365 nm, and the total analysis time was ∼7 min. Method validation was accomplished by examination of the linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ). More importantly, in a linear range of 0.15–10 mg L−1, OTC had high repeatability with good peak shape and no irreversible adsorption was found. The results of this study demonstrated that bare silica column could be used in the determination of tetracycline antibiotics in HILIC mode with no peak tailing, providing a different understanding from that previously found in literature.
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