Abstract
Previous studies have suggested the prerequisite of bone interfacing successful endosteal dental implants. This is the concept of osseointegration of dental implants within the jaw. However, the ultrastructure of the actual bone interface is largely unknown. This current study is planned to elucidate with correlative high-voltage electron microscopy (HVEM) and conventional electron microscopy (TEM), the morphology of the interface of the support tissues to endosteal dental implants. All ultrastructural protocols involve undecalcified tissue sectioning.After 2 months healing following extraction of all premolars, 120 root form and blade-type endosteal dental implants were placed into the mandibles of 30 adult mongrel dogs. This report will be restricted to 12 implants retrieved from animals euthanized after 5 months of unloaded healing. Following perfusion with buffered 3% glutaraldehyde the implants were block resected with their encasement of mandibular tissues, fixed by immersion for 24 hr in glutaraldehyde and postfixed with 1% OsO4 for 2 hr.
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