HPTLC-Densitometric Method for Simultaneous Estimation of Olmesartan medoxomil and Chlorthalidone in Tablet Dosage Form

Abstract

The principle motive behind this work is the densitometric high-performance thin-layer chromatography (HPTLC) method has been developed and validated for the simultaneous estimation of Olmesartan medoxomil and Chlorthalidone in three different brands of the tablet dosage form. The method involves silica gel 60 F254 high-performance thin-layer chromatography and densitometric detection at 238 nm using Toluene: Ethyl acetate: Methanol: Glacial acetic acid (5:4.7:0.3:0.1 % v/v/v/v) as a mobile phase. The calibration curve was linear over a range of 100-1200 ng/band for Olmesartan medoxomil and 62.5-750 ng/band for Chlorthalidone. Precision provided relative standard deviations (RSD) lower than 2 % of both analytes. Percent recoveries were performed and found to be 99.53-99.90 % for Olmesartan medoxomil and 98.18-99.49 % for Chlorthalidone. Validation of the method was carried out as per International Conference on Harmonization (ICH) guideline Q2 (R1). The HPTLC densitometric driven method is highly suitable for analysis of Olmesartan medoxomil and Chlorthalidone in dosage form without any interference.