Abstract
Aim of this study was to develop a HPTLC fingerprint profiles for various secondary metabolites of methanol extract of the root of the traditional medicinal plant, Naregamia alata. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software were used. HPTLC finger printing of methanol extract of root carried out in three different mobile phases, which showed different Rf values. HPTLC finger printing of methanol extract of root in Mobile phase1 revealed 10 peaks with Rf values in the range of 0.13 to 0.92, Mobile phase 2 showed 7 peaks with Rf values in the range of 0.27 to 0.93 and Mobile phase 3 revealed 4 peaks with Rf values in the range of 0.25 to 0.78. It can be concluded that different Rf values of various phytochemicals provide valuable idea regarding their polarity and selection of solvents for separation of phytochemicals. This study will help in future for identifying this plant for further research.
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