HPTLC FINGERPRINTING OF QUERCETIN FROM FICUS BENGHALENSIS

Abstract

Introduction Ficus benghalensis, a member of Moraceae family has a folkloric plant used since ancient time. This plant has numerous medicinal properties include anti-diabetic, anti-inflammatory, anti-oxidant, anti-tumor, anti-diarrheal, hypolipidemic along with wound healing potential. The aim of the present study is to analyze the ethanolic extract of F.benghalensis by using HPTLC fingerprinting. Material & Methods: Qualitative phytochemical screening and HPTLC fingerprinting (using CAMAG LINOMAT 5, CAMAG REPROSTAR 3, a photo-documentation chamber, CAMAG TLC SCANNER 3 and Hamilton syringe) of ethanolic extract of F.benghalensis leaves was done. The mobile phases are flavonoids and phenols, sample is the dried powder of Banyan leaf extract and standard solution is quercetin. Result: Preliminary qualitative phytochemical screening revealed the presence of alkaloids, glycosides, tannins, phenolic compounds in the ethanolic extract of plant’s leaves. The densitogram showed 13 bands of Rf values, in which peak no 5 with Rf value 0.49 affirmed the presence of quercetin in the ethanolic extract of Ficus benghalensis compared to the standard. Conclusion: HPTLC fingerprinting is reliable, accurate, cost-effective method ideally suitable for analysis, quantification and identification of botanical and herbal medicinal products which could have better future prospects.