Abstract
The medicinal value of a plant lies in the phytoconstituents present in it. These phytochemical compounds form the base of modern drugs. The aim of the present study is to identify the phytochemical constituents present in the traditional medicinal plant Pueraria tuberosa and to develop HPTLC fingerprint profile of acetone extract. Preliminary phytochemical screening was done to identify the phytoconstituents and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5applicator, TLC scanner 3, Reprostar 3 and WINCATS-1.4.3 software were used. The present study revealed the presence ofcarbohydrates, proteins alkaloids, flavonoids, saponins, phenols and tannins in various extracts. The HPTLC fingerprint analysis of acetone extract of Pueraria tuberosa showed 10 peaks at 254nm. The components with Rf values 0.05, 0.21 and 0.72 were predominant with the percentage area of 34.52, 16.16 and 10.10respectively. The preliminary phytochemical analysis revealed the presence of various phytochemicals, which were confirmed by the HPTLC fingerprint profile
Highlights
The medicinal value of a plant lies in the phytoconstituents present in it
The aim of the present study is to identify the phytochemical constituents present in the traditional medicinal plant Pueraria tuberosa and to develop High performance Thin Layer chromatography (HPTLC) fingerprint profile of acetone extract
CAMAG make HPTLC system equipped with Linomat 5applicator, TLC scanner 3, Reprostar 3 and WINCATS-1.4.3 software were used
Summary
The medicinal value of a plant lies in the phytoconstituents present in it. These phytochemical compounds form the base of modern drugs. The aim of the present study is to identify the phytochemical constituents present in the traditional medicinal plant Pueraria tuberosa and to develop HPTLC fingerprint profile of acetone extract. The present study revealed the presence of carbohydrates, proteins alkaloids, flavonoids, saponins, phenols and tannins in various extracts. The HPTLC fingerprint analysis of acetone extract of Pueraria tuberosa showed 10 peaks at 254nm. For the present investigation the medicinal plant Vidari The collected samples were washed thoroughly in running tap water and shade dried under room temperature Such plant used in traditional medicine as fertility 2.1. Preparation of extracts control agent, aphrodisiac, cardiotonic, diuretic, antihyperglycemic, anti hyperlipidemic etc. [2,3,4]
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