Abstract
Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn2+ in aqueous solution. The biosensor designed was able to determine Zn2+ in the range of 0.35 - 12 mg/L with a detection limit of 7.5 μg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.
Highlights
Powerful analytical techniques, such as atomic absorption spectroscopy, isotope dilution inductively coupled plasma mass spectrometry, inductively coupled plasma optical emission spectrometry and voltammetric methods, are widely used and are commercially available for detecting heavy metals ions
The interaction between horseradish peroxidase (HRP) and maize tassel (MT)-MWCNT was evaluated with the FT-IR spectra of HRP, HRP/MTMWCNT film and MT-MWCNT composite film
The FTIR results indicated that the structure of HRP remained almost unchanged in MT-MWCNT composite
Summary
Powerful analytical techniques, such as atomic absorption spectroscopy, isotope dilution inductively coupled plasma mass spectrometry, inductively coupled plasma optical emission spectrometry and voltammetric methods, are widely used and are commercially available for detecting heavy metals ions These techniques exhibit high sensitivity, selectivity, reliability, and accuracy. Zinc contagion can lead to a flulike condition known as metal fever and can be a danger to unborn and newborn children through blood or milk of their mothers when they have absorbed large concentrations of zinc For these reasons, there is a considerable interest in the development of highly sensitive, selective, rapid and reliable analytical methods in their detection. Enzyme based biosensors represent an alternative method to quickly detect trace metal ions through inhibition They possess advantages such as minimum sample pretreatment, low cost and less time of analysis, and sufficient sensitivity and selectivity [6,7,8]. The mode of inhibition was investigated using the Dixon and Cornish-Bowden plots
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