Abstract

Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5’ AAT GGA TTT ATC TGC TCT TCG 3’) specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is based on short oligonucleotide DNA probe immobilized onto zinc oxide nanowires (ZnONWs) chemically synthesized onto gold electrode via hydrothermal technique. The morphology studies of the ZnONWs, performed by field emission scanning electron microscopy (FESEM), showed that the ZnO nanowires are uniform, highly dense and oriented perpendicularly to the substrate. Recognition event between the DNA probe and the target was investigated by differential pulse voltammetry (DPV) in 0.1 M acetate buffer solution (ABS), pH 7.00; as a result of the hybridization, an oxidation signal was observed at +0.8 V. The influences of pH, target concentration, and non-complimentary DNA on biosensor performance were examined. The proposed DNA biosensor has the ability to detect the target sequence in the range of concentration between 10.0 and 100.0 μM with a detection limit of 3.32 μM. The experimental results demonstrated that the prepared ZnONWs/Au electrodes are suitable platform for the immobilization of DNA.

Highlights

  • IntroductionThat affects mainly inner lining of milk ducts, is one of the major causes of death of our times

  • Breast cancer, that affects mainly inner lining of milk ducts, is one of the major causes of death of our times

  • Breast cancer has been associated to various gene mutations but the most common mutations, all associated to the activation of breast cancer cells, are Breast cancer 1 (BRCA1), BRCA2 and p53 which occurred in the error-correcting mechanisms

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Summary

Introduction

That affects mainly inner lining of milk ducts, is one of the major causes of death of our times. The main objective of the work reported was to demonstrate that ZnO nanowires, chemically grown onto Au substrate, could provide a suitable platform for the development of electrochemical DNA biosensor for the detection of (BRCA1) gene. This concept could be further elaborated into array format for the multiplexed detection of other breast cancer genes such as BRCA2, and p53 and can be applied on real samples of breast cancer cells

Instrumentation
Reagents
Hybridization of ssDNA
Results and Discussion
Effect of DNA Target Loading on DNA Biosensor Response
Dependence of Biosensor Response on pH
Calibration Characteristic and the Stability of DNA Biosensor
Conclusions
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