Histomorphological changes in induced benign prostatic hyperplasia with exogenous testosterone and estradiol in adult male rats treated with aqueous ethanol extract of Secamone afzelii

Abstract

Secamone afzelii (S. afzelii) used locally to manage benign prostatic hyperplasia (BPH) was used to treat exogenously induced BPH in adult male Wister rats. Male rats weighing 200±10gkg−1 had exogenous administration of testosterone and estradiol in staggered doses (three times weekly) for three weeks. The induced animals were in five groups (6 rats per group). Groups 1 and 2 received extract at 200 and 400mgkg−1 body weight (bwt) by gavages for thirty days; group 3, finasteride (0.1mgkg−1); group 4, untreated for thirty days; group 5, negative control, which was sacrificed twenty-one days after induction. Group 6 received extract (400mgkg−1) and steroid hormones simultaneously; group 7, normal control. The extract caused marked decrease in prostate weight of BPH induced rats with the photomicrograph of the prostate showing extensive shrinkage of glandular tissue whereas glandular hyperplasia occurred in the negative control. Prostate specific antigen (PSA) level significantly (p<0.05) decreased in the treated groups compared to negative control. Treatment with the extract/finasteride caused significant decrease in testosterone to a level comparable to normal. The BPH induced rats treated with S. afzelii/finasteride recorded marked increase in the levels of antioxidant enzymes compared to the negative control. S. afzelii effectively ameliorated prostatic hyperplasia exogenously induced by causing extensive shrinkage of glands and stroma. It also exhibited antioxidant properties and showed to be a good prophylaxis.