Abstract
High-performance thin-layer chromatography with densitometric detection has been used for quantitative analysis of chlorogenic acid in extracts obtained by different methods from the fruits of Peucedanum alsaticum . Separation was achieved on silica gel HPTLC plates with ethyl acetate-formic acid-acetic acid-water 100:11:11:21 as mobile phase. Densitometric detection was performed before and after derivatization with Naturstoffreagenz A at 320 nm. Calibration plots were linear in the range 10–75 μg mL −1 . The results obtained were very similar to those from HPLC, so this densitometric method can be easily used for easy and economic quantitative analysis of active compounds in plant material.
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