Quantitative HPTLC analysis of phenylpropanoids inRhodiolaspecies

Abstract

A simple and rapid HPTLC method with densitometric detection has been established for analysis of rosin, rosarin, and rosavin, three marker compounds from the roots of Rhodiola rosea L. and Rhodiola sachalinensis Borissova. The method was successfully used for quantitative evaluation of commercial samples. Separation on silica gel HPTLC plates was achieved with chloroform-methanol-water 65:35:10.5 (ν/ν) as mobile phase. After development, plates were observed under UV light at 250 nm. The method was validated for linearity, precision, accuracy, and limits of detection (LOD) and quantification (LOQ). The calibration plots were linear in the range 100 to 500 ng per band with all correlation coefficients >0.999. Recovery of the three compounds was between 97 and 101%. The LOD and LOQ were 30 and 100 ng per band, respectively, for all three compounds. The rosin, rosarin, and rosavin content of Rhodiola rosea L. was 0.059, 0.238, and 0.421% (w/w), respectively. Rhodiola sachalinensis Borissova contained 0.034 ...