Abstract
An automatic analyser based on high-performance liquid chromatography has been developed for the quantification of biogenic guanidino compounds in human physiological fluids. Fourteen guanidino compounds are mutually separated within 35 min on a cation-exchange column with a stepwise gradient elution of pH and/or ionic strength in the mobile phase and then converted automatically to their fluorescent derivatives with benzoin. The method in this system is simple, rapid and sensitive; the lower limits of detection are 5–50 pmol for monosubstituted guanidino compounds, 1 nmol for creatine and 20 nmol for creatinine in 100 μl of injection volume.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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