Abstract
A high-performance liquid chromatographic method is described for the simultaneous separation of nine guanidino compounds of biological importance. Guanidino compounds are converted into the corresponding fluorescent derivatives by reaction with benzoin, and separated within 25 min on a reversed-phase column, μBondapak Phenyl, with linear gradient elution using aqueous methanol containing a Tris—hydrochloric acid buffer (pH 8.5). The method is simple, rapid and sensitive; the lower limits of detection for the guanidino compounds are 20–100 fmol in a 100-μl injection volume.
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