Highly sensitive electrochemical detection of dopamine and uric acid on a novel carbon nanotube-modified ionic liquid-nanozeolite paste electrode

Abstract

A novel biosensor has been constructed by incorporating modified nanosized natural zeolite and 3-hydroxypropanaminium acetate (HPAA) as a novel room temperature ionic liquid, supported on multiwalled carbon nanotube (MWCNTs) and employed for the simultaneous determination of dopamine (DA) and uric acid (UA). A detailed investigation by transmission electron microscopy and electrochemistry is performed in order to elucidate the preparation process and properties of the composites. The voltammetric studies using the modified carbon paste electrode show two well-resolved anodic peaks for DA and UA with a potential difference of 160 mV, revealing the possibility of the simultaneous electrochemical detection of these compounds. The modified carbon paste electrode shows good conductivity, stability, and extraction effect due to the synergic action of HPAA, MWCNTs, and iron ion-doped natrolite zeolite. Under optimized conditions, the peak currents are linear from 8.12 × 10−7 to 3.01 × 10−4 mol L−1 and from 9.31 × 10−7 to 3.36 × 10−4 mol L−1 with detection limits of 1.16 × 10−7 and 1.33 × 10−7 mol L−1 for DA and UA using the differential pulse voltammetric method, respectively. Finally, the modified carbon paste electrode proved to have good sensitivity and stability and is successfully applied for the simultaneous determination of DA and UA in human blood serum and urine samples.