Abstract
Sugarcane bagasse (SCB), a major waste of sugar industry, provides a huge potential for cellulosic ethanol production. Efficient pretreatment method is essential to promote its bioconversion. In this study, a novel and cost-effective pretreatment “Densifying Lignocellulosic biomass with Chemicals followed by Autoclave (DLCA)” was applied on SCB for the first time. Sulfuric acid (sa) was used as the reagent. The effects of pretreatment conditions (temperature, time, acid dosage, solid biomass loading) were investigated. High solid-loading enzymatic hydrolysis and fermentations were conducted. The results showed that DLCA(sa)-SCB owned high enzymatic digestibility and high fermentability. Simultaneous saccharification and co-fermentation (SSCF) was also investigated on DLCA(sa)-SCB. A high ethanol titer of 77.51 g/L (nearly 10%, v/v) and a high ethanol yield of 234.09 g per kg SCB were obtained at 30% solid loading by SSCF with fed-batching biomass and enzymes. This is the highest ethanol titer reported on SCB without washing or detoxification.
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