Combination of high solids loading pretreatment and ethanol fermentation of whole slurry of pretreated rice straw to obtain high ethanol titers and yields

Abstract

In cellulosic ethanol production using lignocellulose, an increase in biomass solids loading during the pretreatment process significantly affects the final ethanol titer and the production cost. In this study, pretreatment using rice straw at high solids loading (20% (w/v)) was evaluated, using maleic acid as a catalyst. After pretreatment at optimal conditions of 190°C, 20min, and 0.2% or 5% (w/v) maleic acid, the highest enzymatic digestibility obtained was over 80%. Simultaneous saccharification and fermentation (SSF) of the whole slurry of pretreated rice straw in the presence of activated carbon to separate inhibitory compounds generated a high ethanol yield of 62.8%, based on the initial glucan in unpretreated rice straw. These findings suggest that high solids loading pretreatment using maleic acid and SSF of the whole slurry of pretreated rice straw can be combined to improve the process economics of ethanol production.