High-throughput and specific detection of microorganisms by intelligent modular fluorescent photoelectric microbe detector

Abstract

Food safety has emerged as a major global issue. Detecting foodborne pathogenic microorganisms and controlling them is vital to guard against foodborne diseases caused by microorganisms. However, the current detection methods need to meet the demand for real-time detection on the spot after a simple operation. Considering unresolved challenges, we developed an Intelligent Modular Fluorescent Photoelectric Microbe (IMFP) system containing a special detection reagent. This IMFP system can automatically monitor microbial growth in which the photoelectric detection, temperature control, fluorescent probe, and bioinformatics screen are integrated into one platform and employed to detect pathogenic microorganisms. Moreover, a specific culture medium was also developed, which matched the system platform for Coliform bacteria and Salmonella typhi. The developed IMFP system could attain a limit of detection (LOD) of about 1 CFU/mL for both bacteria, while the selectivity could reach 99%. In addition, the IMFP system was applied to detect 256 bacterial samples simultaneously. This platform reflects the high-throughput needs of fields for microbial identification and related requirements, such as the development of pathogenic microbial diagnostic reagents, antibacterial sterilization performance tests, and microbial growth kinetics. The IMFP system also confirmed the other merits, such as high sensitivity, high-throughput, and operation simplicity compared to conventional methods, and it has a high potential as a tool for application in the health and food security fields.