Abstract
Diabetes Mellitus (DM) is caused by the pancreas insufficient produce of insulin or the body cells not responding properly to the blood insulin, which leads to disorder of carbohydrate metabolism. When the body has deficient insulin, tissues cannot take glucose in from blood stream. As an alternative metabolic pathway, burning fatty acids produces ketones (β-hydroxybutyric acid: 3-HB) into bloodstream as well as create energy. Too much concentration of blood ketone body is called ketoacidosis, and it can cause serious disturbance of consciousness, or result in death at the worst case. Therefore it is important to measure blood ketone body concentration and control it. Standard method of measuring blood ketone is venous blood test. Therefore, there is many problems such as 1) difficulty of periodic measurement, 2) risks of infection, 3) pain of injection, 4) waiting time for a measurement result. In this study, we developed a novel enzymatic reactor and H2O2 electrode aiming at the application to measure urine/sweat ketone body non-invasively. To evaluate properties of trial product sensors, we prepared test flow circuit which consisted of continuous flow pump, manual injector, and incubator (30/37°C). Within phosphate buffer solution, different concentration (0.5-113.83 [mM]) of ketone body which mixed in 1.0mL/min of continuous flow path could be detected with high accuracy.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call