Abstract
Crenobacter cavernae CAVE-375 and Oxalobacteriaceae sp. strain CAVE-383, two Gram-negative bacteria, were isolated during the first microbiology survey performed in a karst cave in Portugal. We report here the release of their high-quality draft genome sequences, which will be useful for geographic-ecological purposes and the description of novel taxa.
Highlights
Crenobacter cavernae CAVE-375 and Oxalobacteriaceae sp. strain CAVE383, two Gram-negative bacteria, were isolated during the first microbiology survey performed in a karst cave in Portugal
Both strains were grown on nutrient agar (NA) at 25°C, and genomic DNA was extracted with an NZY microbial gDNA isolation kit (NZYTech, Portugal)
For strain CAVE-383, 10,289,999 high-quality raw sequences were assembled into 11 contigs, an N50 value of 1,195,780, and a total genome size of 4,119,171 bp (4.2 Mb)
Summary
Crenobacter cavernae CAVE-375 and Oxalobacteriaceae sp. strain CAVE383, two Gram-negative bacteria, were isolated during the first microbiology survey performed in a karst cave in Portugal. Both strains were grown on nutrient agar (NA) at 25°C, and genomic DNA was extracted with an NZY microbial gDNA isolation kit (NZYTech, Portugal). Prepared using the Nextera XT library prep workflow (Illumina), and 2 ϫ 150-nucleotide (nt) paired-end reads were generated on an Illumina MiSeq instrument.
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