Abstract
Lung cancer has been the most common cancer and the main cause of cancer-related deaths worldwide for several decades. PTGR1 (prostaglandin reductase 1), as a bifunctional enzyme, has been involved in the occurrence and progression of cancer. However, its impact on human lung cancer is rarely reported. In this study, we found that PTGR1 was overexpressed in lung cancer based on the analyses of Oncomine. Moreover, lentivirus-mediated shRNA knockdown of PTGR1 reduced cell viability in human lung carcinoma cells 95D and A549 by MTT and colony formation assay. PTGR1 depletion led to G2/M phase cell cycle arrest and increased the proportion of apoptotic cells in 95D cells by flow cytometry. Furthermore, silencing PTGR1 in 95D cells resulted in decreased levels of cyclin-dependent protein kinase complex (CDK1, CDK2, cyclin A2, and cyclin B1) by western blotting and then PTGR1 is positively correlated with cyclin-dependent protein by using the data mining of the Oncomine database. Therefore, our findings suggest that PTGR1 may play a role in lung carcinogenesis through regulating cell proliferation and is a potential new therapeutic strategy for lung cancer.
Highlights
Lung cancer is still one of the major public health problemsE and the main risk factor of cancer-related deaths worldwide.There were an estimated 1.825 million new cases (12.9% of the total cancers), with nearly one death out of every fiveRcases (1.59 million deaths, 19.4% of the total) in 2012 [1, 2]
PTGR1 Is Highly Expressed in NSCLC Tissues
The relative expression levels of PTGR1 gene in NSCLC were systematically assessed by inquiring the Oncomine database
Summary
Lung cancer is still one of the major public health problemsE and the main risk factor of cancer-related deaths worldwide.There were an estimated 1.825 million new cases (12.9% of the total cancers), with nearly one death out of every fiveRcases (1.59 million deaths, 19.4% of the total) in 2012 [1, 2]. Lung cancer is still one of the major public health problems. E and the main risk factor of cancer-related deaths worldwide. There were an estimated 1.825 million new cases (12.9% of the total cancers), with nearly one death out of every five. Rcases (1.59 million deaths, 19.4% of the total) in 2012 [1, 2]. In. Human PTGR1 (prostaglandin reductase 1) gene is named ZADH3 (zinc binding alcohol dehydrogenase domain containing 3) and LTB4DH (leukotriene B4 12-hydroxydehydrogenase), which was first cloned and identified from kidney cDNA libraries by Yokomizo et al [5]. PTGR1 gene encodes a protein named LTB4DH or 15-oxoprostaglandin 13-reductase, which is a dual-functional enzyme capable of. There were 652,842 new lung cancer cases and 597,182 catalyzing the oxidation of LTB4 and the reduction of 15-. PTGR1 has been shown to be involved in the regulation of
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