Abstract
Enhancing the viability of lyophilized microbial strains by modulating their physiological metabolism is an important focus of microbiological research. Certain amino acids have been shown to influence the physiology of microbial strains; however, their effect on strain survival during lyophilization remains largely unexplored. In this study, we employed five different amino acids to investigate the potential correlation between amino acid supplementation and the viability of Lactiplantibacillus plantarum during lyophilization. We discovered that the addition of 0.05 g/L L-cysteine could significantly improve the post-lyophilization viability of all four L. plantarum strains after culture. Subsequently, the mechanisms underlying this enhancement were elucidated using L. plantarum LIP-1 as a type strain. The results showed that L. plantarum metabolised L-cysteine to produce glutathione, which in turn decreased the levels of reactive oxygen species, enhanced hydroxyl radical scavenging, inhibited the oxidation of unsaturated fatty acids, and maintained cell membrane fluidity. As a result, cell membrane damage was attenuated, ultimately improving the lyophilization resistance of L. plantarum.
Published Version
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