Abstract
The metabolic origin of hypertonically induced intracellular free amino acid (FAA) accumulation in the freshwater pulmonate snail, Helisoma trivolvis was examined. The changes in FAA patterns in response to hypertonic stress were common in haemolymph and tissues; but FAA concentrations were much lower in the former. Transport experiments with 3H-glycine and detection by high-performance liquid chromatography (HPLC) showed that isolated kidneys in vitro took up FAA against a concentration gradient. Fluorography demonstrated that injected 14C-amino acids were incorporated into haemolymph protein significantly less in hypertonically stressed snails than in control snails. These results suggest that, in H. trivolvis, reduction in synthesis of haemolymph protein and FAA transport from haemolymph into cells contribute to intracellular FAA increase during the initial stage of hypertonic stress.
Published Version
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