Abstract

Free amino acids in tea leaves are important chemical constituents that considerably influence the quality of tea, especially that of green tea. The present study was conducted to investigate the effects of the nutrients potassium, magnesium and sulphur on biomass production and amino acid accumulation in tea leaves in pot and field experiments. The results show that biomass production and the free amino acid contents in tea leaves increased considerably following the application of potassium and magnesium. Combined application of both nutrients produced the highest increment in free amino acids. The increased free amino acid content in tea leaves was accompanied by enhanced nitrate reductase activity. This indicates that potassium and magnesium application improved the nitrogen metabolism, leading to an increased synthesis of amino acids. A comparison between K 2 CO 3 vs K 2 SO 4 and MgO vs MgSO 4 revealed significantly higher amino acid contents in tea leaves when both potassium and magnesium were applied in the sulphate form, indicating that not only potassium and magnesium but also sulphur plays an important role in the accumulation of amino acids in tea leaves. A pot experiment using KCl and K 2 SO 4 was carried out in order to compare the two commercially available potassium fertiliser forms in their effect on biomass production and the content of free amino acids in tea leaves. The results showed no difference on biomass production between the two fertiliser forms. However, whereas KCl tentatively depressed nitrate reductase activity and hence the accumulation of free amino acids, K 2 SO 4 significantly increased the contents of these quality promoting constituents of tea leaves. The role of fertiliser composition, detrimental effects of accompanying anions, eg Cl - and differences in nutrient availability (eg oxide sulphate form) and possible impact on soil chemistry as main reasons for the observations, being crucial aspects with regard to fertilisation of tea for good quality are discussed.

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