Abstract
To screen the growth-promoting strains of rhizobia for Ormosia henryi and to establish a good symbiotic nitrogen-fixing system, root nodules of O. henryi collected from Guizhou, Zhejiang, Anhui, Fujian, and Jiangxi Provinces were isolated and identified. Using randomized block design, 20 strains of rhizobia that passed reinoculation and 16SrDNA sequence analysis were inoculated into one-year-old aseptic potted O. henryi seedlings followed by measurement of the photosynthetic rate, transpiration rate, chlorophyll fluorescence parameters, growth indexes, and nodulation indexes. Results showed that the nodulation rates of the 20 strains were above 90.0%, the nodule number and nodule weight of strain No. 7 was the highest. Compared to the control which was not been inoculated, inoculation rhizobium significantly (P < 0.05) increased photosynthetic rate, transpiration rate and chlorophyll fluorescence parameters of O. henryi seedlings. Rhizobium also promoted the growth of above ground and underground parts as well as accumulation of total biomass. Seedlings with different rhizobium strains increased the photosynthetic rate (41.6%-197.4%), transpiration rate (3.2%-512.9%), actual photosynthetic quantum yield (4.8%-95.2%), total root surface area (3.4%-866.1%), average root diameter (6.7%-193.3%), root number (0.5%-92.1%), seedling height growth (6.3%-273.8%), diameter growth (10.7%-185.7%), and biomass (37.9%-310.3%). The comprehensive evaluation showed that the best five strains for promoting growth of O. henryi seedlings were No. 15, 16, 7, 18, and 17, and these could be used as excellent strains for inoculation of O. henryi seedlings.
Published Version
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