Abstract
In this work, using Macrotyloma Uniflorum leaves an affordable and eco-friendly, ZnO and Ag-ZnO metal oxide nanoparticles were reported. Ensuing ZnO and Ag-ZnO nanoparticles were characterized by UV–visible spectroscopy, FT-IR Spectroscopy, X-ray diffraction, Scanning Electron Microscopy with Energy Dispersive Spectroscopy, Transmission Electron Microscopy, and Dynamic Light Scattering. The green synthesized ZnO and Ag-ZnO nanoparticles comprise an average size of about 120.16 nm and 91.17 nm respectively. The minimum inhibitory concentrations (MIC) of these ZnO and Ag-ZnO nanoparticles and mixtures thereof, Ag-ZnO, were determined on B.subtilis, Streptococci and E.coli cultures. MIC and their antimicrobial activity were studied in vitro; both types of nanoparticles showed high antibacterial activity. Also, it has shown excellent results with MIC value of 62.5 µg/ml for antibacterial activity against ZnO and Ag-ZnO nanoparticles. The Ag-ZnO nanoparticles were shown better antimicrobial effect than the ZnO nanoparticles. So, we can strongly suggest these green synthesized nanoparticles as a potent agent for biological applications.
Highlights
Metal oxides are an important class of engineered nanoparticles, because they are widely used in cosmetics and sunscreens, self-cleaning coatings and textiles [1,2]
The unique surface Plasmon resonance (SPR) property of noble metals strongly induces the absorption of the incident light and it can be monitored by UV-visible spectroscopy
No structural alteration is observed on the addition of Ag to Zinc oxide (ZnO), the peak corresponding to that of ZnO is shifted to a longer wavelength with the addition of Ag dopant which probably may result in a decreased energy gap
Summary
Metal oxides are an important class of engineered nanoparticles, because they are widely used in cosmetics and sunscreens, self-cleaning coatings and textiles [1,2]. Et al, in 2005 worked on the II-IV groups (CdSe, ZnS and CdTe) and IV-VI groups (PbSe and PbS) nanoparticles in brain extracellular space (ECS) [4]. Et al, in 2013 observed the reduction of hazardous waste generation and antimicrobial activities using biosynthesized Ag and Zn metal nanoparticles [5]. Our interest is to diverse from applied science ranging from material science to biotechnology. We observed that some metal oxides suits for their particular applications. That is because of their distinctive physicochemical properties of metal oxides and these properties are mainly depending on size and surface to volume ratio of the nano materials [7,8]
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