Abstract
Silver nanoparticles (AgNPs) ranging in size from 1-100 nm show good application potential in many medical fields (therapies, medical devices, molecular diagnostics) due to their antimicrobial properties. The purpose of this study is to characterize from physicochemical perspective the colloidal dispersion obtained through phyto-synthesis. The existence of colloidal silver particles was visually highlighted through Thyndall effect. The bio-reduction of silver ions was analyzed through modern techniques, UV-VIS spectrophotometry and Hyperspectral Microscopy. After getting the colloidal dispersion, its antibacterial activity was proved by sowing on different plates the following types of pathogenic agents: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The UV-VIS spectrum for the methanolic extract with 10% H. perforatum L. and for the silver colloidal dispersions was achieved, observing a max at 455 nm. The hyperspectral images were achieved observing the shape, conformation, and the size of the obtained particles. During the antibacterial efficacy testing on those three strains of pathological agents, in all situations, the colloidal dispersion had a promising antimicrobial effect.
Highlights
Silver nanoparticles (AgNPs) ranging in size from 1-100 nm show good application potential in many medical fields due to their antimicrobial properties
In comparison with the silver ions, the antimicrobial activity of the colloidal silver particles is influenced by the size of the particles, their size being inversely proportional with the antimicrobial effect, due to their increased reactivity determined by the big ratio surface to volume [19]
In the case of the synthesis based on plant use, a range of different vegetal products from a variable number of plant species were proven to be able to generate nanoparticles [26]
Summary
Silver nanoparticles (AgNPs) ranging in size from 1-100 nm show good application potential in many medical fields (therapies, medical devices, molecular diagnostics) due to their antimicrobial properties. The nanoparticles from noble metals have been the target of many researches concerning their unique electronic, optical, mechanical, magnetic and chemical properties Their specific properties are different due to their dimension and the size of their specific surface [4,5,6]. In the case of the synthesis based on plant use, a range of different vegetal products (root, stem, bark, fruits, seeds, latex, gums) from a variable number of plant species were proven to be able to generate nanoparticles [26]. These phyto-syntheses are considered green syntheses [1]
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