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Abstract
The aim of the present study is to search out nontoxic silver nanoparticles synthesized from the leaf extract of two plants Ajuga parviflora Benth and Digera muricata for antimicrobial activity. The plants used in this investigation are rich in alkaloids, flavonoids, steroids, terpenoids, protein, amino acids, carbohydrate, quninones, phenols and tannins. The formation of nanoparticles were confirmed by UV/Visible spectroscopy, peaks at 423nm for Ajuga parviflora Benth and 408nm for Digera muricata. The morphology of the silver nanoparticles was established through state of the art spectroscopic tools. SEM analysis reveals average size of AgNPs 18 nm for Digera muricata and 22 nm for Ajuga parviflora Benth respectively while transmission electron microscopy confirms that AgNPs are spherical in shape. The synthesized nanoparticles were subjected to Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa. The results suggest that the silver nanoparticles have promising activity against all the bacterial strains and can be used an effective bactericides.
Highlights
Nanotechnology is a multidimensional field which interfaces the physical, chemical, medical, and environmental sciences
2.2 Preparation of Ajuga parviflora Benth and Digera muricate leaf extracts Fresh leaves of Ajuga parviflora Benth and Digera muricata were washed with tap water to remove dust followed by double distilled water in order to remove any kind of ions sourced by dust or tap water from the leaf surface
3.1 UV/Visible spectroscopy The change in colour of aqueous solution indicates synthesis of Ag-NPs, which was confirmed from the UV–Vis spectra of aqueous colloidal solution
Summary
Nanotechnology is a multidimensional field which interfaces the physical, chemical, medical, and environmental sciences. This versatile field has numerous applications in the engineering fields; some prominent applications are progress of biosensors and biomedical devices, alternate energy production and ecological restoration [1]. Nanoparticles serve as the basic building blocks for a variety of nanotechnology applications. Nanoparticles are considered as distinct group of chemical materials with unique features and wide applications in various arenas [2]. Nanoparticles of different metals are being used in number of fields which include mechanical, pharmaceutical, electrical, biological, chemical fields and in field of textiles
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