Study of synergistic effect of copper and silver nanoparticles with 10% benzalkonium chloride on Pseudomonas aeruginosa

Abstract

ObjectiveOne of the purposes of nanotechnology is the use of copper and silver nanoparticles as a novel approach in the treatment of microbial infections. This study was conducted to evaluate the synergistic effect of copper and silver nanoparticles with 10% benzalkonium chloride to evaluate their antibacterial properties on Pseudomonas aeruginosa (ATCC27853). Materials and methodsIn this study, silver and copper nanoparticles were commercially available. Pseudomonas aeruginosa (ATCC27853) was used as a test microorganism. Comparison of Antibacterial activity of nanoparticles with benzalkonium was investigated using agar (disk diffusion) and serial dilution. ResultsIn this study, stokes of copper and silver nanoparticles with 10% Benzalkonium chloride solvent had bactericidal effect at different concentrations in comparison with MIC level, the higher concentration had the more antibacterial effects. Moreover, the results showed that silver nanoparticles with 10% Benzalkonium chloride showed more inhibitory effects on Pseudomonas aeruginosa. ConclusionThis study showed that copper and silver nanoparticles have a significant effect on bacterial agents and their composition with 10% benzalkonium chloride disinfectant can have more antibacterial effect on Pseudomonas aeruginosa and, as a result, it can be used as a suitable disinfectant. According to the results, it can be concluded that the anti-bacterial efficacy of 10% benzalkonium chloride can enhance the antimicrobial activity of silver and copper nanoparticles.