An insight study on HPTLC fingerprinting of Mukia maderaspatna: Mechanism of bioactive constituents in metal nanoparticle synthesis and its activity against human pathogens.

Abstract

The present work reports the development of a Simple, rapid and eco-friendly route for the biosynthesis of gold and silver nanoparticles and their antimicrobial activity against gram positive and gram negative bacterial pathogens in the wound infected area. The synthesis was done using Mukia maderasapatna plant extract as a reducing agent. FT-IR analysis reported that the methanol extract was found to contain maximum number of functional groups which is responsible for the reduction of metal ions to metal nanoparticles. Further, the presence of bioactive molecules present in the methanol extract was confirmed by GC-MS analysis. The major phytochemical constituents observed in this Mukia maderaspatna leaf extract were n-hexadecanoic acid (15.51%), Octadecanoic acid (11.92%), stigmasterol (9.12%), Octadecanoic acid, 2, 3 hydroxypropyl ester (8.7%), Bicycloheptane, 2,6,6- trimethyl (5.57%) and stearic acid (4.23%). Initially, the formation of gold and silver nanoparticles was confirmed from their absorption spectra at 535nm and 434nm using UV- visible spectroscopy. The crystalline nature of the obtained gold and silver nanoparticles was confirmed by X-ray diffraction analysis. The average size of the gold and silver nanoparticles found around 50nm and 20nm respectively was confirmed by TEM analysis. The antimicrobial activities of silver and gold nanoparticles were checked against bacterial strains and the fungal strains. The formation of inhibition zones concluded that both the nanoparticles showed efficient antimicrobial activity against both the pathogens. Further, different concentrations of gold and silver nanoparticles, the antibiotics Ciprofloxacin and chloramphenicol as the positive control were carried out. The results concluded that the silver nanoparticles showed efficient activity against bacterial pathogens and fungal pathogens with the lowest concentration of 0.35μg/ml and 0.45μg/ml respectively.