Abstract
Green synthesis route has been used for preparation of silver nanoparticles (AgNPs) by aqueous extract of chard plant (Beta vulgaris). The extract volume was studied as effected parameter in the synthesis of AgNPs and monitored by UV-visible spectrum according to the surface plasmon resonance band that centered at 417 nm. The silver nanoparticles were characterized by FTIR, XRD and SEM. The FTIR anlysis showed the adsorption of biomolecules on the AgNPs surface acting as reducing and capping agent. The XRD results revealed the presence of cubic face silver metal without trace of another silver oxides, as an indication of stability against oxidation. Also XRD in combination with SEM analysis showed that AgNPs are in the nanometric scale with spherical identical shape, where the route was successful. The silver nanoparticles tested against Streptococcus pneumonia and Escherichia coli, where the inhibition zone was 28 and 33 nm, respectively.
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