Green synthesis and antibacterial activity of silver nanoparticles from the aqueous extracts of Cassia alata

Abstract

The current study involves the synthesis of silver nanoparticles from the aqueous extracts of Cassia alata. The synthesized silver nanoparticles were characterized by UV-Vis, FT-IR, SEM-EDX and XRD. The in vitro bioactivity studies were examined against a Gram-positive and Gram-negative micro-organism by performing antibacterial activity, minimum inhibition assay, swarming motility and protein leakage assay. The characteristic study on the nanoparticles using spectrometric and microscopic analysis, revealed them to be sized between 25 nm and 60 nm. The nanoparticles on evaluating the antibacterial activity against Pseudomonas aeruginosa and Bacillus subtilis interacted well with the microorganisms producing a significant inhibitory effect at a minimum concentration of 0.5 µg/mL. The permeability of the particles was also studied through protein leakage assay.