Abstract
In this study, a novel sort of sample preparation sorbent was developed, by preparing thin layer graphene oxide tablets (GO-Tabs) utilizing a mixture of graphene oxide and polyethylene glycol on a polyethylene substrate. The GO-Tabs were used for extraction and concentration of omeprazole (OME) in human saliva samples. The determination of OME was carried out using liquid chromatography-tandem mass spectrometry (LC–MS/MS) under gradient LC conditions and in the positive ion mode (ESI+) with mass transitions of m/z 346.3→198.0 for OME and m/z 369.98→252.0 for the internal standard. Standard calibration for the saliva samples was in the range of 2.0–2000 nmol L−1. Limits of detection and quantification were 0.05 and 2.0 nmol L−1, respectively. Method validation showed good method accuracy and precision; the inter-day precision values ranged from 5.7 to 8.3 (%RSD), and the accuracy of determinations varied from −11.8% to 13.3% (% deviation from nominal values). The extraction recovery was 60%, and GO-Tabs could be re-used for more than ten extractions without deterioration in recovery. In this study, the determination of OME in real human saliva samples using GO-Tab extraction was validated.
Highlights
Omeprazole (OME) is a well-known drug that is used as a proton pump inhibitor to reduce the amount of acid produced in the stomach
Gas chromatography (GC) and liquid chromatography (LC) with mass spectrometric (MS) detection have been used for the determination of OME, and solid phase extraction (SPE) has often been utilized as a sample preparation technique for extracting OME from plasma samples [3,4]
During the last two decades, several sample preparation techniques have been developed and directed towards automation and on-line coupling. Two of these techniques are microextraction by packed sorbent (MEPS) and molecularly imprinted polymer tablets (MIP-Tabs), both of which were developed and introduced by researchers in the Abdel-Rehim group
Summary
Omeprazole (OME) is a well-known drug that is used as a proton pump inhibitor to reduce the amount of acid produced in the stomach. Developing a simple and novel sample preparation technique for measuring the OME in saliva samples is a demanding issue. During the last two decades, several sample preparation techniques have been developed and directed towards automation and on-line coupling Two of these techniques are microextraction by packed sorbent (MEPS) and molecularly imprinted polymer tablets (MIP-Tabs), both of which were developed and introduced by researchers in the Abdel-Rehim group. In our recent work, reduced graphene oxide (GO) materials as a solid phase in the MEPS method, was successfully applied for extraction and measurement of local anesthesia in plasma and saliva samples [18]. GO-Tabs is a novel, straightforward and effective sample preparation trend which will be presented here for extraction of OME from saliva samples and measurement by LC–MS/MS
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