Global gene expression profile of identified neurogliaform interneurons in the neocortex

Abstract

Event Abstract Back to Event Global gene expression profile of identified neurogliaform interneurons in the neocortex E. Boldog1, N. Faragó2, M. Rózsa1, E. Vámos1, S. Oláh1, V. Szemenyei1, S. Lovas1, L. Puskás2 and G. Tamás2 1 University of Szeged, Department of Physiology, Anatomy and Neuroscience, Hungary 2 Biological Research Centre of the Hungarian Academy of Sciences, Hungary Neurogliaform cells (NGFCs) are cortical GABAergic interneurons characterized by α-actinin 2, GABAAd receptor content and unitary volume transmission leading to slow GABAA and GABAB receptor mediated responses. Such functional distinction is expected to be based on the concerted action of several genes active specifically in NGFCs. We analyzed the global gene expression profile of NGFCs and applied a harvesting procedure standardized for single cell PCR. Combining whole cell recordings and post hoc light microscopic assessment to identify NGFCs (n=30) from rats (P22-40), RNA from harvested cytoplasms was amplified and hybridized onto a rat DNA-microarray. Out of 26209 genes, we identified 1143 transcripts that exhibited significantly (p<0.001) higher expression in NGFCs compared to controls. In addition, we tested the expression profile of 40 out of the 1143 NGFC specific transcripts with quantitative real-time PCR thus validating the gene chip analysis. Among the NGFC specific transcripts, we confirmed known markers for NGFCs (GABAAd, α-actinin 2), and in addition, neurotransmitter receptor subtypes undetected in NGFCs to date. Whole cell electrophysiological experiments and immunocytochemistry confirmed the action of dopamine receptor subtypes not reported previously in NGFCs.These initial results confirm that global gene expression profiling combined with functional characterization is routinely feasible in identified cortical interneurons. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Boldog E, Faragó N, Rózsa M, Vámos E, Oláh S, Szemenyei V, Lovas S, Puskás L and Tamás G (2011). Global gene expression profile of identified neurogliaform interneurons in the neocortex. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00098 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers E. Boldog N. Faragó M. Rózsa E. Vámos S. Oláh V. Szemenyei S. Lovas L. Puskás G. Tamás Google E. Boldog N. Faragó M. Rózsa E. Vámos S. Oláh V. Szemenyei S. Lovas L. Puskás G. Tamás Google Scholar E. Boldog N. Faragó M. Rózsa E. Vámos S. Oláh V. Szemenyei S. Lovas L. Puskás G. Tamás PubMed E. Boldog N. Faragó M. Rózsa E. Vámos S. Oláh V. Szemenyei S. Lovas L. Puskás G. Tamás Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.