Abstract
<p>Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.</p>
Highlights
Plant cultures in vitro are seen as an alternative source of natural substances normally acquired from ground plants, that are used as medicines in the prevention of selected diseases or being components of a diet because of their speciic pro-health properties [7, 28]
This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects
The dynamic of growth of P. quinquefolium L. was studied in Murashige and Skoog (MS) medium with 2.4D 1 mg l-1, kin 0.1 mg l-1 and 30 g l-1 saccharose
Summary
Plant cultures in vitro are seen as an alternative source of natural substances normally acquired from ground plants, that are used as medicines in the prevention of selected diseases or being components of a diet because of their speciic pro-health properties [7, 28]. M Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake lasks The bioreactor was inoculated with cell suspension from shake lask cultures after 14 days of cultivation
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More From: Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
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