Abstract
Most medicinal plants are on the verge of extinction. In this regard, biotechnology is facing the challenge of developing alternative ways to produce biomass with the desired Biological active substance. The maximum yield of high growth performance of the cell culture mainly depends on the selection of optimal ratios and concentrations of growth regulators. This problem, namely the search for the optimal composition of the nutrient medium has become one of the main tasks in the cultivation of H. maracandicum Popov ex Kirp plant cells. Methods and results of seed sterilization of H. maracandicum are discussed in the article. This endemic, rare species of medicinal plant from the flora of Uzbekistan family Asteraceae Dumotr has a unique composition of secondary metabolites. For example, from the biomass of immortelle were isolated flavonoids, coumarins, lipids, phenols, purines, steroids, triterpenoids, glycosides, coumarins, cerines, bitter tannins, essential oils, etc. Used in folk medicine for cholecystitis and diseases of the liver, bladder, and gastrointestinal tract.
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