Abstract
Abstract The emergence of Staphylococcus aureus and its ability to confer cross-resistance to clindamycin has complicated the treatment and increase the possible ratio of morbidity and mortality. There are numerous potential causes resulted in the variations in the resistance rate. This study aimed to detect the molecular basis for clindamycin resistance among multidrug resistant Staphylococcus aureus local isolates. According to previous results of our work in press, polymerase chain reaction technique were used to test the existence of ermABC and msrA genes in ten of the clindamycin multidrug resistant S. aureus isolates using specific primers. Molecular detection revealed the presence of the virulence genes (ermABC and msrA genes) in all of the tested S. aureus isolates and the results of PCR amplification for spa gene in S. aureus isolates showed bands of different sizes (250–350 bp). After sequencing and comparing sequences to the spa genotyping database, the results indicated that there were six different spa typing, including t042 (2 isolates; 20%), t304 (1 isolate; 10%), t044 (2 isolates; 20%), t037 (2 isolate; 10%), t18072 (1 isolate; 10%) and t1028 (1 isolate; 10%). Depending on the types of spa produced, the number of repetitions might range from four (t1028) to nine (t304 and t18072). Spa types t042 and t044 were the most prevalent among the studied isolates. ermABC and msrA distribution may be increased as a result of the excessive use of clindamycin make Staphylococcus aureus resistant to it.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call