Abstract
Introduction. In present, the medical community continues to study the impact of coronavirus infection on various organs and systems of the body. Infection with the SARS-CoV-2 virus causes multifaceted pathological processes and, among other things, affects the cells of the circulatory system. Objective: To assess the degree of DNA damage in peripheral blood lymphocytes of patients infected with the SARS-CoV-2 virus. Material and methods. 200 patients took part in the study. The control group consisted of conditionally healthy individuals matched by gender and age (n=50). The degree of DNA damage was assessed using the DNA comet assay in an alkaline medium. The assessment parameters included: tail length (TL) (pc), percentage of damaged DNA in the tail (Tail DNA, %), tail moment (conventional units) and Olive moment (conventional units). Results. The average values of the parameters of comet DNA of patients in the acute period of coronavirus infection were: TL 95.18±5.7 pc, percentage of damaged DNA in the tail (Tail DNA%) 70.82±7.12%, tail moment 68.52±8.58 conventional units, Olive moment 41.11±4.46 conventional units. When comparing the parameters of comet DNA of lymphocytes of conditionally healthy individuals and patients in the acute period of coronavirus infection associated with the SARS-CoV-2 virus, a significant increase (p < 0.001) in these parameters is noted, which indicates an increase in the content of fragmented and damaged DNA in peripheral blood lymphocytes in sick individuals. Conclusion. The obtained results prove the powerful genotoxic effect of the SARS-CoV-2 virus on human cells
Published Version
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