Abstract
ABSTRACT In this report, Acinetobacter calcoaceticus Aca13 was isolated from crude oil-polluted soil. The optimization of growth conditions showed that Acinetobacter calcoaceticus Aca13 can tolerate a wide range of temperatures (14–42°C). The whole-genome sequence showed that the circular chromosome of Acinetobacter calcoaceticus Aca13 was 3,966,524 bp in size, with an average GC content of 38.78%. Whole-genome analysis revealed that 39 cytochrome proteins, 49 monooxygenases and 46 dioxygenases might be related to hexadecane and naphthalene degradation. Whole-transcriptome analysis revealed that six dioxygenases could be induced by naphthalene and that one monooxygenase could be significantly induced by hexadecane. Both genomic and transcriptional results showed that alkane 1-monooxygenase is the key enzyme for hexadecane degradation and that aromatic ring-hydroxylating dioxygenase is the key enzyme for naphthalene degradation. The present study contributes to the genomic and transcriptomic databases of crude oil-degrading microorganisms.
Published Version
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