Genome-wide identification of the TIFY gene family in three cultivated Gossypium species and the expression of JAZ genes

Quan Sun,Peng Qiao,Guanghao Wang,Youlu Yuan,Xiangrui Zhang,Yingfan Cai,Lu Long,Xiao Zhang

doi:10.1038/srep42418

Quan Sun, Peng Qiao + Show 6 more

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https://doi.org/10.1038/srep42418

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Abstract

TIFY proteins are plant-specific proteins containing TIFY, JAZ, PPD and ZML subfamilies. A total of 50, 54 and 28 members of the TIFY gene family in three cultivated cotton species—Gossypium hirsutum, Gossypium barbadense and Gossypium arboretum—were identified, respectively. The results of phylogenetic analysis showed that these TIFY genes were divided into eight clusters. The different clusters of gene family members often have similar gene structures, including the number of exons. The results of quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed that different JAZ genes displayed distinct expression patterns in the leaves of upland cotton under treatment with Gibberellin (GA), methyl jasmonate (MeJA), Jasmonic acid (JA) and abscisic acid (ABA). Different groups of JAZ genes exhibited different expression patterns in cotton leaves infected with Verticillium dahliae. The results of the comparative analysis of TIFY genes in the three cultivated species will be useful for understanding the involvement of these genes in development and stress resistance in cotton.

Highlights

In Arabidopsis, the PPD protein interacts with STERILE APETALA (SAP)[10] and KIX8/911 and plays a role in the regulation of tissue growth, modulation of lamina size, and limitation of the curvature of the leaf blade[12,13]
To identify TIFY-related proteins in cotton genomes, the HMMER profile was implemented to identify the genomes of Gossypium hirsutum, Gossypium barbadense and Gossypium arboreum databases
The results showed that a total of 28 GaTIFY, 54 GbTIFY and 50 GhTIFY genes were characterized from cotton databases

Summary

Introduction

In Arabidopsis, the PPD protein interacts with STERILE APETALA (SAP)[10] and KIX8/911 and plays a role in the regulation of tissue growth, modulation of lamina size, and limitation of the curvature of the leaf blade[12,13]. JAZ proteins play important roles in stress responses, disease resistance, and plant development. In cotton (Gossypium hirsutum), GhJAZ1 plays an important role in maintaining the balance between defence responses against Verticillium wilt and cotton growth[24]. Studies have shown that JAZ genes play an important role in disease biotic and abiotic stress and hormonal signalling pathways[25,26,27,28,29,30,31,32,33]. We successfully discovered the expression patterns of the JAZ proteins in cotton plants in response to hormone treatment and Verticillium dahliae infection. The present study lays the foundation for the further identification of common signalling pathways controlled through JA and other hormones and the mechanisms underlying stress and disease resistance in cotton

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