Genome-wide identification and functional analysis of the TIFY gene family in response to drought in cotton.

Caixiang Wang,Fuguang Li,Yun Song,Hamama Islam Butt,Eryong Chen,Chaojun Zhang,Qianhua Wang,Zuoren Yang,Zhao Liu,Xueyan Zhang,Ge Zhao

doi:10.1007/s00438-016-1248-2

Caixiang Wang, Fuguang Li + Show 9 more

Open Access

https://doi.org/10.1007/s00438-016-1248-2

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Abstract

Jasmonates control many aspects of plant biological processes. They are important for regulating plant responses to various biotic and abiotic stresses, including drought, which is one of the most serious threats to sustainable agricultural production. However, little is known regarding how jasmonate ZIM-domain (JAZ) proteins mediate jasmonic acid signals to improve stress tolerance in cotton. This represents the first comprehensive comparative study of TIFY transcription factors in both diploid A, D and tetraploid AD cotton species. In this study, we identified 21 TIFY family members in the genome of Gossypium arboretum, 28 members from Gossypium raimondii and 50 TIFY genes in Gossypium hirsutum. The phylogenetic analyses indicated the TIFY gene family could be divided into the following four subfamilies: TIFY, PPD, ZML, and JAZ subfamilies. The cotton TIFY genes have expanded through tandem duplications and segmental duplications compared with other plant species. Gene expression profile revealed temporal and tissue specificities for TIFY genes under simulated drought conditions in Gossypium arboretum. The JAZ subfamily members were the most highly expressed genes, suggesting that they have a vital role in responses to drought stress. Over-expression of GaJAZ5 gene decreased water loss, stomatal openings, and the accumulation of H2O2 in Arabidopsis thaliana. Additionally, the results of drought tolerance assays suggested that this subfamily might be involved in increasing drought tolerance. Our study provides new data regarding the genome-wide analysis of TIFY gene families and their important roles in drought tolerance in cotton species. These data may form the basis of future studies regarding the relationship between drought and jasmonic acid.Electronic supplementary materialThe online version of this article (doi:10.1007/s00438-016-1248-2) contains supplementary material, which is available to authorized users.

Highlights

Cotton is the most important fiber and oil crop worldwide, and its production is threatened by abiotic stresses, such as drought and salinity stresses (Grayson 2013)
We report the analysis of the TIFY genes from Gossypium arboretum, Gossypium raimondii and Gossypium hirsutum pertaining to genomic organization, gene structures and the phylogenetic tree analysis
The transgenic plants recovered from the drought conditions much better than the WT plants (Fig. 6). These results suggested that GaJAZ5 affects drought tolerance

Summary

Introduction

Cotton is the most important fiber and oil crop worldwide, and its production is threatened by abiotic stresses, such as drought and salinity stresses (Grayson 2013). Breeding for drought-resistant cotton should be a high priority for plant biotechnology programs Phytohormones, such as auxin, gibberellin, and abscisic acid, considerably affect plant growth, development, aging, dormancy, and stress resistance. It regulates plant responses to various biotic and abiotic stresses, such as wounding, UV light, water deficit, pathogens, and ozone It is involved in a wide range of plant developmental processes, including pollen and stamen development, vegetative growth control, anthocyanin accumulation, and senescence (Conconi et al 1996; McConn and Browse 1996; Creelman and Mullet 1997; Rao et al 2000; Berger 2002; Schommer et al 2008; Cheng et al 2009; Ren et al 2009; Qi et al 2011; Wang et al 2013)

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