Abstract
The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) gene family comprises plant-specific transcription factors that control cell proliferation and differentiation during growth and development in many plant species. However, to date, no studies of the LBD gene family in Dendrobium catenatum have been reported. In this study, a genome-wide analysis of LBD genes was performed in D. catenatum and 24 LBD genes were identified. The genes were classified into two classes (I and II) based on phylogenetic relationships and motif structure. Subcellular localization analysis for DcaLBD6 and DcaLBD18 from class I and DcaLBD37 and DcaLBD41 from class II revealed that the proteins were localized in the nucleus. Transient expression analysis of DcaLBD6, DcaLBD18, DcaLBD37, and DcaLBD41 indicated that class I and class II members have opposite roles in regulating VASCULAR-RELATED NAC-DOMAIN 7 (VND7) expression. DcaLBD genes showed diverse expression patterns in response to different phytohormone treatments. Heat maps revealed diverse patterns of DcaLBD gene expression in different organs. These results lay the foundation for further detailed studies of the LBD gene family in D. catenatum.
Highlights
The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors play important roles in the growth and development of many plant species
High, andstudies the numb have shown that the LBD gene family members are absent in Chlamydomonas reinhardtii shows a trend expanding from scratch
Eucalyptus grandis LBD genes differ in degree of influence on secondary growth; overexpression of EgLBD37 results in a significant increase in secondary xylem development, whereas overexpression of EgLBD29 leads to a significant increase in phloem fiber development [34]. These results suggest that DcaLBD37 and DcaLBD41 might be involved in xylem formation via regulation of the expression of VASCULAR-RELATED NAC-DOMAIN 7 (VND7)
Summary
The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors play important roles in the growth and development of many plant species. LBD genes are involved in the initiation, growth, metabolic regulation, and secondary growth of roots, stems, leaves, and corollas, establishment of the boundary between lateral organ and terminal meristem primordia, and have an important influence on the formation and development of aboveground and belowground organs in higher plants. The LOB domain comprises a C-block, which contains conserved cysteine residues in the CX2CX6CX3C motif required for DNA-binding activity. All LBD proteins contain the C-block, but LBD proteins of class I include a leucine zipper structure, whereas class II proteins lack a complete leucine zipper structure and cannot form a spiral coil [7,8,9]
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