Abstract
Single nucleotide polymorphism (SNP) may influence the genesis and development of cancer in a variety of ways depending on their location. Here we conducted a study in Chinese female non-smokers to investigate the relationship between rs1049337, rs926198 and the risk or survival of lung cancer. Further, we explored whether rs1049337 could alter the binding affinity between the mRNA of CAV1 and the corresponding microRNAs. Finally, we evaluated the relationship between expression level of CAV1 and prognosis of lung cancer. The results showed that the rs1049337-C allele and rs926198-C allele were the protective alleles of lung cancer risk. Haplotype analysis indicated that the C-C haplotype (constructed by rs1049337 and rs926198) was a protective haplotype for lung cancer risk. The result of luciferase reporter assay showed that rs1049337 can affect the binding affinity of CAV1 mRNA to the corresponding microRNAs both in A549 cell line and H1299 cell line. Compared with C allele, T allele had a relatively decreased luciferase activity. Compared with paired normal adjacent tissue or normal lung tissue, lung cancer tissue showed a relatively low level of CAV1. Refer to those patients at early stage of lung cancer, the expression level of CAV1 in patients at late stage of lung cancer was relatively low. In conclusion, the results indicated that rs1049337, it's a SNP located at 3′UTR region of CAV1 may affect lung cancer risk by altering the binding affinity between the mRNA of CAV1 and the corresponding microRNAs.
Highlights
IntroductionAs the poor 5-year survival rate, lung cancer has become the leading cause of death among females in more developed countries and males in the whole world
Lung cancer is the most frequently diagnosed lethal malignancies in the world
We conducted a study to explore the relationship between CAV1 gene polymorphism, expression level and lung cancer
Summary
As the poor 5-year survival rate, lung cancer has become the leading cause of death among females in more developed countries and males in the whole world. As is known to all, smoking is regarded as a common risk factor of lung cancer. Global cancer statistics state that 15% of men and 53% of women with lung cancer were not caused by smoking [1]. Smoking rates in Chinese women are lower than some European countries, but the incidence of lung cancer in China is relatively high [2]. This suggested us that some other factors may be involved in lung cancer in Chinese female population
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